Journal: Oncogene
Article Title: CD146 is a novel marker for highly tumorigenic cells and a potential therapeutic target in malignant rhabdoid tumor
doi: 10.1038/onc.2016.72
Figure Lengend Snippet: CD146 expression in MRT cell lines and primary tumors. ( a ) MP-MRT-AN, KP-MRT-NS, KP-MRT-RY and KP-MRT-YM cells were stained with anti-CD146 (black histograms) or isotype-matched control antibodies (gray histograms), and then analyzed by flow cytometry. ( b ) Representative flow cytometric profile of ATRT primary tumor cells stained with anti-CD146 and anti-CD133 antibodies, showing that a small CD146 + sub-population was present, whereas CD133 + cells were rarely observed.
Article Snippet: MRT cell lines were treated with 0.05% trypsin/EDTA (Life Technologies), and sorted cells were resuspended in serum-free α-minimum essential medium for experiments.
Techniques: Expressing, Staining, Control, Flow Cytometry